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Specific polypeptide markers in chronic B cell malignancies detected by two‐dimensional gel electrophoresis
Author(s) -
TalebianZiai Simin,
Azgui Zahia,
Valensi Françoise,
Sigaux François,
Charron Dominique
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130179
Subject(s) - chronic lymphocytic leukemia , hairy cell leukemia , polyacrylamide gel electrophoresis , b cell , lymphoma , microbiology and biotechnology , biology , leukemia , immunology , antibody , biochemistry , enzyme
Abstract Two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) analysis of silver stained polypeptides was used to discriminate the cell protein profiles in chronic B cell malignancies. We identified 5 distinct polypeptides (H1 to H5) present in hairy cell leukemia (HCL) (14 cases) and absent in most chronic lymphocytic leukemia (CLL) and normal B lymphocytes. Two polypeptides, H2 and H5, were absolutely specific of HCL. The 2‐D PAGE profiles found in 3 splenic lymphoma with villous lymphocytes (SLVL) and 2 HCL variants (HCL‐V) were similar to HCL with a unique additional peptide present in 3/3 SLVL and 1/2 HCL‐V. When HCL and SLVL were assessed for the CLL stage‐specific 2‐D PAGE patterns, HCL was related to stage A/B while SLVL was related to stage C. In conclusion, 2‐D PAGE analysis of the molecular pattern of B lymphocytes provides a new means to recognize the leukemic origin of the sample and distinguish HCL from CLL and SLVL.