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The analysis of human serum transferrins with the PhastSystem: Quantitation of microheterogeneity
Author(s) -
van Eijk Hendrik G.,
van Noort Willem L.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130173
Subject(s) - transferrin , isoelectric focusing , densitometry , human plasma , immunoprecipitation , chromatography , chemistry , hemopexin , blood proteins , quantitative analysis (chemistry) , antibody , microbiology and biotechnology , biochemistry , biology , immunology , medicine , gene , enzyme , heme
The PhastSystem was used for three different analyses of human serum transferrins (Tf) by isoelectric focusing: (i) The distribution of iron over the two iron binding sites by separation of the four forms: apoTf, TfFe c , Fe N Tf and Fe 2 Tf by using < 1 μL serum or plasma. The focused bands were visualized by immunoprecipitation and quantified by densitometry (ii) The relative proportions of the diferic sialo‐transferrin fractions in human sera or plasma were determined in a similar way. (iii) The presumed genetic variants can be determined. The methods are easy and reproducible and require much less sample (0.3 μL), time (1 h per run) and antibody (10 μL/sample) than crossed immunoelectrofocusing.