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High resolution two‐dimensional gel analysis of proteins in the central nervous system of larvae of Drosophila melanogaster
Author(s) -
Assigeo Rosa,
Santarén Juan F.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130164
Subject(s) - drosophila melanogaster , biology , gel electrophoresis , methionine , imaginal disc , melanogaster , instar , larva , two dimensional gel electrophoresis , drosophilidae , amino acid , polyacrylamide gel electrophoresis , central nervous system , nervous system , strain (injury) , microbiology and biotechnology , biochemistry , anatomy , enzyme , botany , neuroscience , gene , proteomics
An improved method of high resolution two‐dimensional gel electrophoresis was used to study the patterns of protein synthesis in the central nervous system (CNS) of late instar larvae of Drosophila melanogaster . A small number of CNSs was radiolabeled with a mixture of 14 C‐labeled amino acids or with [ 35 S]methionine, and the pattern of labeled proteins was analyzed. One thousand and forty‐five polypeptides, 800 acidic (IEF) and 245 basic (NEPHGE), from the CNS of several wild‐type strains have so far been separated and cataloged. All these polypeptides were numbered and compared with our catalog of polypeptides from wing imaginal discs, and quantitative or qualitative changes were detected in more than 23% of the polypeptides. When comparing patterns of label of CNSs we found small quantitative differences in the rate of synthesis between individuals of the same strain, not due to sexual differences, and a minute number of quantitative and qualitative differences between groups of individuals of different strains.