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Polyacrylamide gel electrophoresis in vertical, inverse and double‐crossing gradients of soluble polymers
Author(s) -
Gersten Douglas M.,
Bijwaard Karen E.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130159
Subject(s) - polymer , polyacrylamide , electrophoresis , polyethylene glycol , chromatography , chemistry , gel electrophoresis , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , sodium , polymer chemistry , biochemistry , organic chemistry , enzyme
The presence of soluble dextrans, methylcellulose and polyethylene glycol polymers incorporated into vertical sodium dodecyl sulfate (SDS)‐polyacrylamide slabs during electrophoresis can have a pronounced effect on protein separations. The effects of various standard and inverse gradients of polymers on the electrophoretic mobility of marker proteins in 10%T, 2.66%C Laemmli‐style SDS gels, and the effects of simultaneous pore size and polymer gradients were investigated. These experiments demonstrate that the inclusion of polymers is a new, additional parameter that can be useful in resolving complex mixtures of proteins.