Polyacrylamide gel electrophoresis in vertical, inverse and double‐crossing gradients of soluble polymers | Zendy

Gersten Douglas M. | Zendy; Bijwaard Karen E. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Polyacrylamide gel electrophoresis in vertical, inverse and double‐crossing gradients of soluble polymers

Author(s) -

Gersten Douglas M.,

Bijwaard Karen E.

Publication year - 1992

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150130159

Subject(s) - polymer , polyacrylamide , electrophoresis , polyethylene glycol , chromatography , chemistry , gel electrophoresis , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , sodium , polymer chemistry , biochemistry , organic chemistry , enzyme

The presence of soluble dextrans, methylcellulose and polyethylene glycol polymers incorporated into vertical sodium dodecyl sulfate (SDS)‐polyacrylamide slabs during electrophoresis can have a pronounced effect on protein separations. The effects of various standard and inverse gradients of polymers on the electrophoretic mobility of marker proteins in 10%T, 2.66%C Laemmli‐style SDS gels, and the effects of simultaneous pore size and polymer gradients were investigated. These experiments demonstrate that the inclusion of polymers is a new, additional parameter that can be useful in resolving complex mixtures of proteins.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research