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Modified silver staining for immobilized pH gradients
Author(s) -
Rabilloud Thierry,
Brodard Virginie,
Peltre Gabriel,
Righetti Pier Giorgio,
Ettori Claudia
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130154
Subject(s) - thiosulfate , molar concentration , silver stain , adsorption , chemistry , staining , anode , molar ratio , concentration gradient , chromatography , cathode , immobilized ph gradient , nuclear chemistry , organic chemistry , electrode , catalysis , sulfur , isoelectric focusing , microbiology and biotechnology , biology , enzyme , medicine , pathology
Silver development of gels containing an immobilized pH gradient has proved difficult so far because the bonded buffers (especially the tertiary amino acrylamido derivatives) tend to adsorb silver ions with a resultant heavy background of increasing darkness from the anode to the cathode. We report a variant of silver staining in which thiosulfate is used twice: (i) prior to silver impregnation, at the millimolar level, to enhance sensitivity, and (ii) during development, at the micro‐molar level, to decrease the background.