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Rapid and simple method for the identification of apolipoprotein E isomorphic phenotypes from whole serum
Author(s) -
Kohlmeier Martin,
Drossel HansJoachim,
Sinha Pranav,
Köttgen Eckart
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130152
Subject(s) - isoelectric focusing , immobilized ph gradient , chromatography , gene isoform , blot , apolipoprotein b , chemistry , immunostaining , electrophoresis , polyacrylamide gel electrophoresis , isoelectric point , phenotype , microbiology and biotechnology , biochemistry , biology , immunology , cholesterol , gene , immunohistochemistry , enzyme
For the identification of apolipoprotein E isomorphic phenotypes, fresh or thawed serum was analyzed without prior delipidation or other pretreatment. Using 5% polyacrylamide gels with a 40 mm interelectrode distance, the isoforms were separated by isoelectric focusing in immobilized pH gradients ranging from pH 5 to 6.5, and transferred onto polyvinylidene difluoride membranes by contact blotting for 1 h. The apolipoprotein E isoforms were identified following immunostaining. The electrophoresis required less than 2 h and the entire procedure could be completed within 6 h.