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Carbohydrate analysis of transferrin subfractions isolated by preparative isoelectric focusing in immobilized pH gradients
Author(s) -
de Jong Gerard,
van Noort Wim L.,
van Eijk Hendrik G.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130146
Subject(s) - isoelectric focusing , transferrin , isoelectric point , chromatography , glycan , chemistry , carbohydrate deficient transferrin , carbohydrate , biochemistry , glycosylation , glycoprotein , enzyme , alcohol , alcohol consumption
Analytical isoelectric focusing of human serum transferrin has revealed the presence of up to nine different transferrin subfractions. The less prevalent subfractions have hitherto not been available for analytical work on their composition. Prolonged isoelectric focusing in immobilized pH gradients is capable of effectively separating and concentrating these subfractions from other fractions that are often present in over 70‐fold excess in native preparations. Preliminary results of the carbohydrate analysis indicate a heterogeneity extending beyond the present concepts of the transferrin glycan structure.