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Comparison of different staining methods for polyvinylidene difluoride membranes
Author(s) -
Christiansen Jesper,
Houen Gunnar
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130137
Subject(s) - staining , coomassie brilliant blue , immunostaining , chemistry , membrane , chromatography , silver stain , biochemistry , microbiology and biotechnology , biology , immunohistochemistry , genetics , immunology
Several new staining methods for polyvinylidene difluoride membranes, including mercurochrome, silver and dimethylaminoazobenzene isothiocyanate staining were compared with Coomassie Brilliant Blue and gold staining. Of these, Coomassie was most versatile and completely compatible with ensuing microsequencing, immunostaining or other visualization methods, while gold and silver staining were more sensitive. Mercurochrome allows selective detection of sulfhydryl‐containing proteins while dimethylaminoazobenzene isothiocyanate staining may allow quantitation of sequenceable protein.