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A rapid polymerase chain reaction method for identifying fixed specimens
Author(s) -
Gill Peter,
Kimpton Cohn P.,
Sullivan Kevin
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130135
Subject(s) - polymerase chain reaction , polymerase chain reaction optimization , chemistry , polymerase , chain (unit) , inverse polymerase chain reaction , computational biology , chromatography , nested polymerase chain reaction , microbiology and biotechnology , biology , biochemistry , dna , gene , physics , astronomy
DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin “Chelex” to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance association using two microsatellites were calculated as ranging between 10 −2 and 10 −4 . The methods described have considerable potential for use in routine genetic analysis of preserved samples.