Premium
Five new alleles of plasma Zn‐alpha 2‐glycoprotein variants phenotyped by isoelectric focusing and immunoblotting in twelve populations
Author(s) -
Nakayashiki Nori,
Umetsu Kazuo,
Yuasa Isao,
Suenaga Kazuyuki,
Omoto Keiichi,
Ishida Takafumi,
Misawa Shogo,
Katsura Syusaku
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130131
Subject(s) - isoelectric focusing , allele , alpha (finance) , glycoprotein , genetics , biology , microbiology and biotechnology , gene , biochemistry , medicine , construct validity , nursing , patient satisfaction , enzyme
Abstract Human Zn‐alpha 2‐glycoprotein (ZAG) in plasma samples from twelve populations was tested by immunoblotting after polyacrylamide gel isoelectric focusing. Eleven ZAG phenotypes produced by one common and nine rare alleles, including five new ones (ZAG*6–ZAG*10), were detected. Additionally, an application of separator IEF with N ′‐2‐hydroxyethylpiperazine‐ N ′‐2‐ethanesulfonic acid (HEPES) was found to be useful for discriminating the rare ZAG 7 band.