Transverse pore gradient gel electrophoresis of lipoprotein [a] using methylenebisacrylamide gradients | Zendy

Smejkal Gary B. | Zendy; Hoff Henry F. | Zendy

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Transverse pore gradient gel electrophoresis of lipoprotein [a] using methylenebisacrylamide gradients

Author(s) -

Smejkal Gary B.,

Hoff Henry F.

Publication year - 1992

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150130121

Subject(s) - transverse plane , monomer , electrophoresis , chemistry , materials science , range (aeronautics) , chromatography , analytical chemistry (journal) , polymer , composite material , anatomy , biology

We describe a modification of transverse pore gradient gel electrophoresis in which pore size is regulated by crosslinker proportion (%C) rather than by total monomer concentration (%T). We electrophoresed plasma lipoprotein [a] transversely across linear N,N ′‐methylenebisacrylamide gradients and measured mobility as a function of %C. This method allows for the simultaneous assessment of pore sizes generated over a wide range of crosslinker proportions.

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