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Transverse pore gradient gel electrophoresis of lipoprotein [a] using methylenebisacrylamide gradients
Author(s) -
Smejkal Gary B.,
Hoff Henry F.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130121
Subject(s) - transverse plane , monomer , electrophoresis , chemistry , materials science , range (aeronautics) , chromatography , analytical chemistry (journal) , polymer , composite material , anatomy , biology
We describe a modification of transverse pore gradient gel electrophoresis in which pore size is regulated by crosslinker proportion (%C) rather than by total monomer concentration (%T). We electrophoresed plasma lipoprotein [a] transversely across linear N,N ′‐methylenebisacrylamide gradients and measured mobility as a function of %C. This method allows for the simultaneous assessment of pore sizes generated over a wide range of crosslinker proportions.