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Localization of separated protein bands in unstained electrophoresed polyacrylamide gradient slab gel
Author(s) -
Arora D. Jit S.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130120
Subject(s) - polyacrylamide gel electrophoresis , gel electrophoresis of nucleic acids , gel electrophoresis , slab , gel electrophoresis of proteins , molecular weight size marker , polyacrylamide , electrophoresis , two dimensional gel electrophoresis , chromatography , difference gel electrophoresis , color marker , pulsed field gel electrophoresis , chemistry , materials science , biology , biochemistry , polymer chemistry , proteomics , paleontology , gene , genotype , enzyme
Polyacrylamide gel electrophoresis has become the most widely used separation method in biological science. Once electrophoresis is complete the protein bands must be localized prior to excision. A zig‐zag gel cutter is described which cuts a strip of gel from the side of a slab gradient gel or a gel of uniform concentration in peaks and valleys. The location of the protein of interest is determined by counting the number of peaks on the stained side strip. The portion of the unstained gel corresponding to the same count (number of valleys) is cut to recover the protein of interest from the main gel for further manipulations.