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A simple and rapid method for HLA‐DQA1 genotyping by polymerase chain reaction‐single strand conformation polymorphism and restriction enzyme cleavage analysis
Author(s) -
Hayashi Tomonori,
Seyama Toshio,
Ito Takashi,
Kusunoki Yoichiro,
Hirai Yuko,
Nakamura Nori,
Akiyama Mitoshi
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301191
Subject(s) - genotyping , polymerase chain reaction , restriction enzyme , locus (genetics) , biology , microbiology and biotechnology , single strand conformation polymorphism , genetics , human leukocyte antigen , allele , cleaved amplified polymorphic sequence , genotype , dna , polymerase , restriction fragment length polymorphism , gene , antigen
A simple and rapid method for identification of alleles at the human leucocyte antigen (HLA)‐DQA1 locus is described. The polymorphic second exon of the HLA‐DQA1 locus was amplified by the polymerase chain reaction (PCR) method. The amplified DNA was analyzed by single‐strand conformation polymorphism (SSCP) and restriction enzyme cleavage assay. Using this method, he eight known DQA1 alleles could be distinguished from each other. This paper suggests that the method can be used for quick genotyping of DQA1 alleles, but detecting point mutations at various positions in a fragment as well as new HLA‐DQA1 genotypes should also be possible.