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A rapid periodic acid‐Schiff staining procedure for the detection of glycoproteins using the phastsystem
Author(s) -
VanSeuningen Isabelle,
Davril Monique
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130119
Subject(s) - isoelectric focusing , isoelectric point , chromatography , stain , staining , chemistry , glycoprotein , neuraminidase , periodic acid–schiff stain , sodium dodecyl sulfate , gel electrophoresis , electrophoresis , periodic acid , biochemistry , biology , genetics , enzyme
A procedure for the analysis of glycoproteins and glycopeptides using the PhastSystem with detection by the periodic acid‐Schiff stain is described. Following sodium dodecyl sulfate or nondenaturing polyacrylamide gel electrophoresis and also isoelectric focusing, samples are stained directly for the presence of carbohydrates. By using the PhastSystem, the method is rapid, sensitive, reliable and allows storage of the gels without a change in the stain. As little as 0.1 μg of protein‐associated carbohydrates can be detected. The staining procedure is also used following isoelectric focusing of mucin‐derived glycopeptides to visualize their charge differences and the increase of their isoelectric point after neuraminidase treatment.