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Identification of Gram‐negative bacteria by isoelectric focusing of soluble cellular proteins
Author(s) -
Margull Andreas,
Süssmuth Roland,
Schulz Peter
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301186
Subject(s) - isoelectric focusing , proteus mirabilis , isoelectric point , escherichia coli , bacteria , polyacrylamide gel electrophoresis , microbiology and biotechnology , pseudomonas aeruginosa , biology , identification (biology) , gel electrophoresis , chromatography , chemistry , biochemistry , genetics , botany , gene , enzyme
Strains of Escherichia coli. Pseudomonas aeruginosa and Proteus mirabilis , isolated from clinical specimens were disrupted by repeated freezing in liquid nitrogen and thawing at room temperature. The samples were separated by isoelectric focusing using polyacrylamide gels. The resulting protein patterns showed clear differences between the three species and made identification possible.