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Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis
Author(s) -
Andreev Victor P.,
Lisin E. E.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301183
Subject(s) - capillary electrophoresis , capillary action , electrophoresis , chemistry , buffer (optical fiber) , electro osmosis , analytical chemistry (journal) , viscosity , electrical resistivity and conductivity , resolution (logic) , chromatography , conductivity , flow (mathematics) , materials science , mechanics , composite material , telecommunications , physics , engineering , artificial intelligence , computer science , electrical engineering
The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.