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Discontinuous electrophoresis of β‐caseins for the determination of bovine caseins in milk and dairy products
Author(s) -
Mayer Walter,
Hörtner Hermann
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301175
Subject(s) - bovine milk , chemistry , casein , electrophoresis , chromatography , milk protein , food science , capillary electrophoresis
Discontinuous acidic anodic polyacrylamide gel electrophoresis enables the separation of bovine β‐caseins from those of ovine and caprine. Interfering protein bands as a consequence of ripening or processing have not been detected. After evaluation of the stained gel by laser densitometry, quantification was performed with calibration standards on the same gel by the ratio of the peak areas from bovine to ovine and bovine to caprine, respectively. Thus, independence from the extractability of proteins affected by denaturation and ripening (which might in some cases raise the limit of detection) is achieved. The range of quantification extends from 5 to approximately 70% bovine casein in relation to total casein.