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The use of radioactive bacteriophage proteins as X–Y markers for silver stained two‐dimensional electrophoresis gels and quantification of the patterns
Author(s) -
Gersten Douglas M.,
Ramagli Louis S.,
Johnston Dennis A.,
Rodriguez Lewis V.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130117
Subject(s) - silver stain , densitometry , staining , electrophoresis , bacteriophage , chromatography , spots , chemistry , microbiology and biotechnology , biology , biochemistry , escherichia coli , genetics , gene , physics , quantum mechanics
Abstract We have demonstrated the feasibility of using bacteriophage ghost proteins, tritiated by metabolic labeling, as a set of standard markers for two‐dimensional gels in which the proteins are to be detected by silver staining. The results indicate that a 2.5 μg load of phage proteins yields a reproducible silver pattern of 48 spots. The spots can also be readily identified by radioautography and radiofluorography, establishing their value as a standard constellation of markers. Quantification of these patterns by computerized densitometry indicates a general agreement between detection by silver staining and detection by radiofluorography.