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Comparison of the cellular protein composition of aortic smooth muscle cells in vivo and in vitro by two‐dimensional gel electrophoresis
Author(s) -
Weiß HansDieter,
Betz Eberhard,
Karsch Karl Rüdiger
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301165
Subject(s) - smooth muscle , in vitro , cell , thoracic aorta , vascular smooth muscle , aorta , microbiology and biotechnology , staining , myocyte , silver stain , two dimensional gel electrophoresis , primary culture , gel electrophoresis , chemistry , anatomy , in vivo , protein expression , immunohistochemistry , biology , biochemistry , immunology , proteomics , medicine , endocrinology , genetics , gene
Vascular smooth muscle cells are of major interest for the evaluation of atherosclerotic processes. By the use of two‐dimensional gel electrophoresis and subsequent silver staining we investigated the protein expression of smooth muscle cells in the native thoracic aorta, immediately after enzymatic disaggregation, in subconfluent proliferating and in postconfluent nonproliferating primary cell cultures. Compared to the native thoracic aorta the protein composition of smooth muscle cells in cell culture is changed dramatically. Furthermore, significant differences in protein expression between proliferating and nonproliferating smooth muscle cells in cell culture were found.