Premium
Application of high resolution two‐dimensional polyacrylamide gel electrophoresis of polypeptides from cultured neonatal rat cardiomyocytes: Regulation of protein synthesis by catecholamines
Author(s) -
He Chang,
Müller Ursula,
Oberthür Walter,
Werdan Karl
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301163
Subject(s) - norepinephrine , polyacrylamide gel electrophoresis , spots , silver stain , isoelectric point , two dimensional gel electrophoresis , staining , gel electrophoresis , isoelectric focusing , microbiology and biotechnology , biology , chemistry , biochemistry , endocrinology , proteomics , enzyme , botany , genetics , gene , dopamine
High resolution two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) was applied to cultured neonatal rat heart muscle cells, incubated for 72 h at 37°C in serum‐free medium, either in the absence or in the presence of 0.1 μ M norepinephrine. After silver staining, about 340 and 550 protein spots could be seen in cardiomyocytes, cultured either in the absence or presence of norepinephrine. Of these spots, 141 could be further characterized according to isoelectric point and molecular weight, with 71 protein spots being present under both conditions. In cells cultivated in presence of norepinephrine, 58 new protein spots appeared, whereas 12 spots disappeared, and 22 spots increased (whereas 3 spots decreased) in intensity. In comparison with 2‐D PAGE of rat cardiomyocytes, the protein pattern of the intact heart of neonatal rats is incongruent. 2‐D PAGE of polypeptides of cultured neonatal rat cardiomyocytes may be a suitable tool to study the regulation of protein synthesis by various stimuli with relevance to cardiac growth adaptation, inotropy and heart failure.