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Using two‐dimensional gel electrophoresis to study immune response against intracellular bacterial infection
Author(s) -
Kovarova Hana,
Stulik Jiri,
Macela Ales,
Lefkovits Ivan,
Skrabkova Zuzana
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301160
Subject(s) - gel electrophoresis , immune system , antigen , biology , electrophoresis , in vivo , two dimensional gel electrophoresis , host response , microbiology and biotechnology , immunology , biochemistry , genetics , proteomics , gene
Principal component analysis was applied to two‐dimensional (2‐D) gel electrophoresis patterns, obtained in various phases of infection. Untreated controls could be satisfactorily differentiated from patterns after infection on days 3 and 7 whereas day 10 of infection was grouped with the controls. Comparison of host cellular protein patterns could help to classify in vivo developing infection without requiring any so‐called immune marker functions. Immunoaffinity separation of infected cells treated with detergent, followed by 2‐D electrophoresis of negative as well as positive eluates, did not reveal radiolabeled bacterial protein antigens.