Identification of human myocard proteins separated by two‐dimensional electrophoresis

N ‐Terminal sequencing, internal sequencing and amino acid analysis were used to identify twelve proteins of the human myocard two‐dimensional gel electrophoresis (2‐DE) pattern. Amino acid analysis was shown to be a powerful tool in addition to sequencing. The identification of a disease‐associated N ‐terminally blocked protein by internal sequencing was not successful. The twelve identified proteins are the basis of a human myocard 2‐DE database.