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Identification of human myocard proteins separated by two‐dimensional electrophoresis
Author(s) -
Jungblut Peter,
Otto Albrecht,
Regitz Vera,
Fleck Eckart,
WittmannLiebold Brigitte
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301159
Subject(s) - identification (biology) , gel electrophoresis , biology , amino acid , computational biology , biochemistry , chemistry , botany
N ‐Terminal sequencing, internal sequencing and amino acid analysis were used to identify twelve proteins of the human myocard two‐dimensional gel electrophoresis (2‐DE) pattern. Amino acid analysis was shown to be a powerful tool in addition to sequencing. The identification of a disease‐associated N ‐terminally blocked protein by internal sequencing was not successful. The twelve identified proteins are the basis of a human myocard 2‐DE database.