Premium
A human myocardial two‐dimensional electrophoresis database: Protein characterisation by microsequencing and immunoblotting
Author(s) -
Baker Cathy S.,
Corbett Joseph M.,
May Andrew J.,
Yacoub Magdi H.,
Dunn Michael J.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301154
Subject(s) - isoelectric point , isoelectric focusing , human heart , electrophoresis , human proteins , database , protein expression , chemistry , microbiology and biotechnology , computational biology , biology , biochemistry , computer science , enzyme , medicine , gene , cardiology
This communication briefly describes how a human heart two‐dimensional electrophoresis (2‐DE) protein database is being established in our laboratory. The database contains more than 1500 polypeptides and approximately fifty proteins from 2‐DE gels of human myocardial tissue have been characterised. Information about the proteins has been compiled including molecular weight ( M r ), isoelectric point (p I ), sample spot (SSP) number, protein name, partial sequence, and antibody reacting with the protein. The first stage of this project involves the investigation of protein with p I s in the range pH 4–7. Future studies will employ immobilised pH gradient (IPG) gels as the first dimension of the 2‐DE to examine basic proteins. The ultimate goal of this project is to establish a global picture of human heart protein expression in both normal and disease conditions.