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Immune complexes in blood: A new strategy for the analysis of their antigen portion
Author(s) -
Fried Roman,
Wiederkehr Felix,
Vonderschmitt Dieter J.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301152
Subject(s) - immune system , antigen , chemistry , computational biology , immunology , biology
A method is described for the characterization of protein antigens from circulating immune complexes from plasma. Free immunoglobulins G were separated from larger immune complexes by gel filtration with a fast protein liquid chromatographic system. The collected immune complexes were dissociated with 4 M urea into antigens and antibodies. With a second column run with 4 M urea, antigens smaller than 120 kDa were separated from unloaded antibody fractions. After concentration, they were analyzed by two‐dimensional gel electrophoresis.