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Pulsed‐field gel electrophoresis analysis of a Pseudomonas aeruginosa pathovar
Author(s) -
Römling Ute,
Grothues Dietmar,
Koopmann Uta,
Jahnke Birgit,
Greipel Joachim,
Tümmler Burkhard
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301134
Subject(s) - pseudomonas aeruginosa , housekeeping gene , pulsed field gel electrophoresis , biology , colonization , gel electrophoresis , pathovar , microbiology and biotechnology , virulence , pseudomonadaceae , genome , genetics , gene , bacteria , genotype , gene expression
This paper presents the genome organization and mobility of Pseudomonas aeruginosa strains that had been isolated in half‐year intervals from 30 patients with cystic fibrosis since the onset of colonization over a 2‐ to 8‐year period. The chromosomes were digested with Dra I or Spe I, separated by pulsed‐field gel electrophoresis, blotted and hybridized with probes encoding housekeeping or virulence genes. Strains were differentiated by relatedness of macrorestriction fingerprints. After some turnover of strains during the first two years of colonization, each patient had acquired a set of strains that diversified during the course of the disease. In the majority of patients, two clonal lineages were found to account for colonization in the air passages but each lung habitat was characterized by some specific signature of bands in the macrorestriction fragment pattern.