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Agarose gel pore radii are not dependent on the casting buffer
Author(s) -
Stellwagen Nancy C.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501301121
Subject(s) - agarose , buffer (optical fiber) , agarose gel electrophoresis , electrophoresis , chromatography , gel electrophoresis , capillary electrophoresis , materials science , chemistry , analytical chemistry (journal) , dna , gel electrophoresis of nucleic acids , buffer solution , biochemistry , telecommunications , computer science
Previous studies have shown that the apparent pore size of agarose gels is dependent on the buffer in which the gel is cast and run (D.L. Holmes and N.C. Stellwagen, Electrophoresis 1990, 11 , 5–15; N.C. Stellwagen and D.L. Holmes, Electrophoresis 1990, 11 , 649–652). However, these studies, based on the mobility of DNA restriction fragments, neglected the effect of electroendosmosis. By measuring the mobility of vitamin B 12 under various experimental conditions, it is shown here that electroendosmosis is highly buffer‐dependent. When the observed mobilities of DNA are corrected for electroendomosis, the apparent pore radii of agarose gels are found to be independent of the casting buffer.