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Ti/IrO 2 as anode and Zr as cathode in multicompartment electrolyzers with Immobiline membranes
Author(s) -
Ettori Claudia,
Righetti Pier Giorgio,
Comninellis Christos
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130111
Subject(s) - isoelectric focusing , anode , membrane , cathode , electrolyte , electrolysis , isoelectric point , electrode , materials science , chromatography , chemistry , chemical engineering , analytical chemistry (journal) , biochemistry , engineering , enzyme
Multicompartment electrolyzers with isoelectric Immobiline membranes are used for large‐scale preparative protein purification. A series of isoelectric membranes, of defined p I values, is utilized for keeping any desired species isoelectric within each compartment of the electrolyzer. It is preferable to have electrode disks of the same surface area as the membranes for a proper performance of the instrument because electrolyte solutions of low conductivity are used. The use of Pt disks would be quite expensive; we therefore propose using Zr as a cathode and Ti/IrO 2 as an anode in the electrodic compartments. This pairing of electrodes seems to give the same performance as Pt wires. Also, conventional isoelectric focusing, as well as isoelectric focusing in immobilized pH gradients, both requiring a good contact area between gel and electrodes, would benefit by using flat laminae of these metals as electrodes.