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Capillary electrophoretic analysis of flavonoids
Author(s) -
Seitz Ulrike,
Oefner Peter J.,
Nathakarnkitkool Suraphol,
Popp Michael,
Bonn Günther K.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130107
Subject(s) - rutin , capillary electrophoresis , chemistry , chromatography , calibration curve , detection limit , capillary action , boron , analytical chemistry (journal) , electrophoresis , flos , resolution (logic) , materials science , organic chemistry , artificial intelligence , computer science , composite material , antioxidant
Combining the effects of electrophoresis and electroendosmosis, flavonoids were separated in less than ten minutes in a fused silica capillary tube with a borate buffer adjusted to pH 10. An increase in the concentration of borate from 0.1 to 0.2 M resulted in longer migration times due to a decrease in electroosmotic flow, but also in improved selectivity and higher resolution of flavonoids. The calibration curve of rutin showed a detection limit of 0.02 mg/mL and linearity over its pharmaceutical concentration range. Using an internal standard of known concentration, the content of rutin in a methanolic extract of Sambuci flos could be determined with a coefficient of variation as small as 3.8% by the molar ratio – peak area ratio method.