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A gel retardation assay system for studying protein binding to simple repetitive DNA sequences
Author(s) -
Mäueler Winfried,
Muller Marc,
Köhne Anja C.,
Epplen Jörg T.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130103
Subject(s) - repeated sequence , dna , biology , simple (philosophy) , genome , genetics , computational biology , major histocompatibility complex , dna sequencing , gene , microbiology and biotechnology , philosophy , epistemology
Simple repetitive DNA sequences have been regarded as mere “junk” present in all eukaryotic genomes. In fact, mixed simple repeat (gt) n (ga) m sequences are present in major histocompatibility complex MHC‐DRB genes for long evolutionary times, including such distant animals as artiodactyla and man. We describe herein an unsophisticated method which reveals that at least certain simple repetitive (gt) n (ga) m sequences bind nuclear proteins and show characteristics of a specific DNA‐protein interaction via gel retardation.