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Effective recovery by KCl precipitation of highly diluted muscle proteins solubilized with sodium dodecyl sulfate
Author(s) -
Carraro Ugo,
Rizzi Corrado,
Sandri Marco
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150121203
Subject(s) - sodium dodecyl sulfate , chemistry , solubility , precipitation , chromatography , solubilization , protein precipitation , sodium , gel electrophoresis , potassium , sulfate , pellet , electrophoresis , biochemistry , extraction (chemistry) , materials science , physics , organic chemistry , composite material , meteorology
A new, improved method is described, which takes advantage of the low solubility of potassium dodecyl sulfate (KDS), to concentrate sodium dodecyl sulfate‐solubilized proteins from nanomolar solutions by KDS precipitation. The method was applied to proteins differing in M r and p I . The effect of varying KCl, and the pH and/or concentration of the buffer on KDS‐protein precipitation was studied. These parameters may be chosen to allow selective repartition of specific proteins in the pellet or supernatant. After precipitation, the hundred‐ or thousand‐fold concentrated solutes are easily resuspended in small volumes of any required medium. Though initially experimented to recover muscle proteins from effluents of electroendosmotic preparative gel electrophoresis, the method proved to be of general interest as a powerful tool to recover proteins from highly diluted solutions.