Premium
A two‐dimensional gel database of rat liver proteins useful in gene regulation and drug effects studies
Author(s) -
Anderson N. Leigh,
EsquerBlasco Ricardo,
Hofmann JeanPaul,
Anderson Norman G.
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150121110
Subject(s) - isoelectric point , sodium dodecyl sulfate , listing (finance) , cholesterol , isoelectric focusing , molecular mass , chemistry , biology , biochemistry , computational biology , finance , economics , enzyme
A standard two‐dimensional (2‐D) protein map of Fischer 344 rat liver (F344MST3) is presented, with a tabular listing of more than 1200 protein species. Sodium dodecyl sulfate (SDS) molecular mass and isoelectric point have been established, based on positions of numerous internal standards. This map has been used to connect and compare hundreds of 2‐D gels of rat liver samples from a variety of studies, and forms the nucleus of an expanding database describing rat liver proteins and their regulation by various drugs and toxic agents. An example of such a study, involving regulation of cholesterol synthesis by cholesterol‐lowering drugs and a high‐cholesterol diet, is presented. Since the map has been obtained with a widely used and highly reproducible 2‐D gel system (the Iso‐Dalt® system), it can be directly related to an expanding body of work in other laboratories.