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Production of high purity proteins by elution of individual fractions from two‐dimensional gels
Author(s) -
Tsvetkova Mari.,
Kovalyov Leonid I.,
Laptev Alexei V.,
Shishkin Sergei S.
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120717
Subject(s) - elution , chromatography , tropomyosin , aqueous solution , chemistry , homogeneous , myosin , potassium , biochemistry , organic chemistry , physics , thermodynamics
Results are presented on the production of purified myocardial proteins from two‐dimensional (2‐D) gels. Proteins were fixed in a native condition using potassium acetate and then eluted into an aqueous solution. Homogeneous tropomyosin and myosin light chain fractions and a number of nonidentified myocardial proteins present on 2‐D gels were obtained.