Premium
The identification of proliferation and tumour‐induced proteins in human endothelial cells: A possible target for tumour therapy
Author(s) -
Clarke Mark S. F.,
West David C.
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120708
Subject(s) - monoclonal antibody , epitope , endothelial stem cell , endothelium , umbilical vein , antigen , biology , antibody , microbiology and biotechnology , chemistry , cancer research , immunology , biochemistry , in vitro , endocrinology
The continued growth and spread of tumours is dependent on the proliferation of the endothelial cells of their vasculature. The presence of proliferation‐ or tumour‐induced surface proteins on these endothelial cells would offer a suitable epitope for monoclonal antibody therapy of tumours. Using cultured human umbilical and capillary endothelial cells, we have stimulated them with simple mitogens and tumour conditioned media and examined the proteins induced by [ 35 S]methionine incorporation and 125 I‐surface‐labelling. Two‐dimensional polyacrylamide gel electrophoresis revealed the induction of proliferation and tumour‐related antigens on the surface of the endothelial cells. Subsequent monoclonal antibody studies suggest that tumour specific surface proteins are present on most tumour endothelium.