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Analysis of circulating immune complexes isolated from plasma, cerebrospinal fluid and urine
Author(s) -
Wiederkehr Felix,
Büeler Martin R.,
Vonderschmitt Dieter J.
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120705
Subject(s) - cerebrospinal fluid , urine , chromatography , size exclusion chromatography , chemistry , antibody , polyethylene glycol , immune system , urinary system , biochemistry , immunology , medicine , pathology , enzyme
The protein nature of soluble immune complexes (IC) from fresh plasma, cerebrospinal fluid (CSF), and urine was studied by combining several analytical and biochemical techniques. In plasma and CSF, free immunoglobulins G were separated from larger IC by gel filtration with a fast protein liquid chromatographic system. In urine, IC were separated by precipitation with polyethylene glycol. IC were further purified by protein‐A and protein‐G affinity chromatography and analyzed by two‐dimensional gel electrophoresis. Apart from plasma samples from healthy donors, IC from cases with macrocreatine kinase type 1 and multiple sclerosis were analyzed. For CSF two cases of multiple sclerosis and for urine one case with urinary tract infection are shown. The method can be used for the examination of IC of unknown protein composition in body fluids.