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A practicable two‐dimensional electrophoresis of urinary proteins as a useful tool in medical diagnosis
Author(s) -
Lapin Alexander,
Feigl Walter
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120704
Subject(s) - electrophoresis , coomassie brilliant blue , sodium dodecyl sulfate , gel electrophoresis , chromatography , gel electrophoresis of proteins , chemistry , staining , medicine , polyacrylamide gel electrophoresis , biochemistry , pathology , enzyme
Practical experience with a rapid two‐dimensional electrophoresis technique for routine analysis of urinary proteins is discussed. The method consists of cellulose acetate electrophoresis in combination with sodium dodecyl sulfate (SDS) electrophoresis, performed together with Coomassie Blue gel staining on “PhastSystem”. Over 400 analyses were performed within the time of two years. Most patients were from nephrological, urological and kidney‐transplant departments. Some of them were from obstetric, pediatric or oncological departments. A systematic discussion and evaluation of the two‐dimensional protein pattern with typical examples is outlined.