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Quantification of proteins in sample buffer for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis using colloidal silver
Author(s) -
Dráber Pavel
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120617
Subject(s) - sodium dodecyl sulfate , nitrocellulose , chromatography , chemistry , silver stain , densitometry , staining , gel electrophoresis , polyacrylamide gel electrophoresis , polyacrylamide , electrophoresis , sodium , sample preparation , membrane , biochemistry , biology , microbiology and biotechnology , enzyme , physics , genetics , organic chemistry , quantum mechanics , polymer chemistry
A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate‐sample buffer is described. Proteins are bound to a nitrocellulose membrane, stained with colloidal silver, and quantified by transmission densitometry. The staining requires 1 μL of protein sample and takes less than 20 min. Good linearity between the staining intensity and amount of proteins is in the range of 5–100 ng.