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Demonstration of murine pancreas elastase and its interstrain variation by isoelectric focusing
Author(s) -
von Deimling Otto H.,
Gaa Andreas,
Hameister Horst
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120614
Subject(s) - isoelectric focusing , proteases , elastase , microbiology and biotechnology , isoelectric point , protease , biochemistry , chromosome , biology , chemistry , chromatography , enzyme , gene
Isoelectric focusing between pH 9 and 11 was used for separation of murine pancreas proteases. One of these proteases is characterized by its preference for N ‐acetyl‐L‐alanine‐α‐naphthylester as substrate and by its genetic linkage to bt , a coat color marker of chromosome 15. This protease was identified as elastase, and is probably elastase‐1 (ELA‐1). Because of the simple procedure and the excellent reproducibility of the focusing pattern, ELA‐1 is recommended as a useful marker for mouse chromosome 15.