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A technique for detecting antifungal activity of proteins separated by polyacrylamide gel electrophoresis
Author(s) -
De Bolle Miguel F. C.,
Goderis Inge J.,
Terras Franky R. G.,
Cammue Bruno P. A.,
Broekaert Willem F.
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120612
Subject(s) - polyacrylamide gel electrophoresis , antifungal , polyacrylamide , chromatography , electrophoresis , gel electrophoresis , chemistry , biochemistry , biology , microbiology and biotechnology , enzyme
A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread on top of the polyacrylamide gel. The position of proteins with antifungal activity can be determined on a diffusion blot prepared from the same gel. The technique is illustrated for three antifungal plant proteins, i.e. α‐purothionin, Urtica dioica agglutinin, and tobacco chitinase.