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Alcian blue fixation allows silver staining of the isolated polysaccharide component of bacterial lipopolysaccharides in polyacrylamide gels
Author(s) -
Corzo Javier,
PérezGaldona Ricardo,
LeónBarrios Milagros,
GutiérrezNavarro Angel M.
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120611
Subject(s) - staining , polysaccharide , silver stain , polyacrylamide , lipopolysaccharide , chemistry , fixation (population genetics) , polyacrylamide gel electrophoresis , cationic polymerization , chromatography , biochemistry , biology , microbiology and biotechnology , polymer chemistry , genetics , gene , enzyme , endocrinology
The effect of the cationic dye Alcian Blue on the silver staining of bacterial lipopolysaccharide and its polysaccharide and lipid A portions in polyacrylamide gels was investigated. The polysaccharide was only stained when the gel was previously treated with the dye. The polysaccharide moiety was found to be responsible for the lipopolysaccharide staining with silver, whereas the lipid A seemed unimportant. Treatment with Alcian Blue may prove useful to detect hydrophilic components of lipopolysaccharide samples that could no be stained by the usual silver staining procedures.