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A rapid (< 10 minute) electrophoresis method for identification of wheat varieties
Author(s) -
Wrigley Colin W.,
Gore Peter J.,
Manusu H. Perry
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120514
Subject(s) - electrophoresis , chromatography , urea , ethylene glycol , chemistry , extraction (chemistry) , biochemistry , organic chemistry
Conventional procedures for electrophoretic identification of gain samples according to variety are too slow to permit checking at the time of delivery. The method described permits electrophoretic identification within an hour. It involves extraction of gliadin proteins from crushed grain with 6% urea solution or ethylene glycol, cathodic electrophoresis for 9 min at 300 V in a Micrograd gel (MG 315 from Gradipore Ltd, Sydney, Australia) using sodium lactate buffer (pH 3.1), and staining in Gradipore (at about 50°C). Distinction between a set of Australian varieties was similar to that obtainable with the Australian Standard Procedure.