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Luminography – a new, highly sensitive visualization method for electrophoresis
Author(s) -
Schneppenheim Reinhard,
Budde Ulrich,
Dahlmann Nicolaus,
Rautenberg Peter
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120508
Subject(s) - chromogenic , luminol , horseradish peroxidase , chemiluminescence , hydrogen peroxide , blot , electrophoresis , chemistry , luminescence , sensitivity (control systems) , protein detection , gel electrophoresis , chromatography , visualization , analytical chemistry (journal) , materials science , biochemistry , nanotechnology , enzyme , computer science , optoelectronics , data mining , electronic engineering , engineering , gene
A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light‐emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X‐ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.