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A reliable two‐dimensional gel electrophoresis procedure for separating neural proteins
Author(s) -
SempleRowland Susan L.,
Adamus Grazyna,
Cohen Robert J.,
Ulshafer Robert J.
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120414
Subject(s) - isoelectric focusing , two dimensional gel electrophoresis , isoelectric point , electrophoresis , computer science , chromatography , gel electrophoresis , resolution (logic) , gel electrophoresis of proteins , chemistry , computational biology , polyacrylamide gel electrophoresis , artificial intelligence , biology , biochemistry , proteomics , gene , enzyme
Abstract A facile two‐dimensional gel electrophoresis procedure has been developed for the analysis of neural tissue proteins which eliminates the serious problems associated with protein insolubility at the point of sample application onto polymerized first dimension isoelectric focusing gels. This was accomplished by combining the methods of two previously published procedures. Our proecedure provides an alternative method to the complex gel systems often employed for less soluble proteins, and yields very reproducible, high resolution separations. This procedure, which is in routing use in our laboratories for the analysis of total proteins extracted from retina and brain, produces protein patterns that are easily compared using both visual and computer‐assisted image analysis techniques. Presented here are the results of a set of experiments designed to identify proteins unique to retina. This procedure should be useful to investigators studying protein changes resulting from genetic mutation, development, drug treatment or disease, in neural tissue as well as in virtually all other tissues.