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Two‐dimensional electrophoresis in small gels for applications in veterinary medicine
Author(s) -
Miller Ingrid,
Gemeiner Manfred
Publication year - 1991
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150120413
Subject(s) - isoelectric focusing , electrophoresis , chromatography , dimension (graph theory) , computer science , chemistry , mathematics , biochemistry , pure mathematics , enzyme
A versatile method for two‐dimensional electrophoresis that can be performed easily, even in small routine laboratories, is described. The procedure combines a first‐dimensional isoelectric focusing run in a PhastSystem with denaturing electrophoresis in a small vertical electrophoresis chamber. The described arrangement of two first‐dimensional gel strips in the second dimension allows direct comparison of two related samples, eliminating most of the artifacts that usually lead to misinterpretations. The presented procedure can be conveniently adapted to the needs of each laboratory; at present, it is being extensively used to establish proteir patterns of healthy individuals of different species, a prerequisite with regard to help and support diagnosis.