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Effects of methanol and urea on optical resolution of phenylthiohydantion‐DL‐amino acids by micellar electrokinetic chromatography with sodium N ‐dodecanoyl‐L‐valinate
Author(s) -
Otsuka Koji,
Terabe Shigeru
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150111121
Subject(s) - micellar electrokinetic chromatography , chemistry , urea , methanol , chromatography , resolution (logic) , enantiomer , sodium , amino acid , electrokinetic phenomena , electrophoresis , stereochemistry , organic chemistry , biochemistry , artificial intelligence , computer science
Effects of methanol and urea on enantiomeric resolution of phenylthiohydantoin (PTH)‐DL‐amino acids by micellar electrokinetic chromatography with sodium N ‐dodecanoyl‐L‐valinate (SDVal) were investigated. PTH‐DL‐Met that could not be optically resolved with an SDVal solution alone was resolved by using an SDVal/methanol solution. Four PTH‐DL‐amino acids were separated from each other and each pair of enantiomers was also optically resolved, although seriously tailed peaks were observed for each solute. The addition of urea to SDVal/methanol solutions improved these peak shapes.