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Conditions for purification of proteins by free‐flow zone electrophoresis
Author(s) -
Clifton Michael J.,
Jouve Nicolas,
Balmann Hélène De,
Sanchez Victor
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150111106
Subject(s) - free flow electrophoresis , electrophoresis , separation (statistics) , residence time (fluid dynamics) , chromatography , flow (mathematics) , diffusion , chemistry , capillary electrophoresis , separation method , mechanics , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , computer science , geology , physics , thermodynamics , biochemistry , geotechnical engineering , machine learning , enzyme
Abstract The separation of proteins by free‐flow zone electrophoresis is generally impaired by a number of secondary effects which cause spreading of the protein streams with resultant loss in resolution. A strategy is outlined, based on experimental observations and numerical modeling, which allows the operating conditions and separation chamber dimensions to be chosen so as to obtain complete separation of two proteins of known mobility. This approach takes into account some dispersive phenomena such as molecular diffusion, electroosmosis and residence time gradients. In some cases, the right operating conditions cannot be achieved on earth and electrophoresis separations in microgravity may then be justified.