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Preparative free‐flow isoelectric focusing: Modeling and experiments
Author(s) -
Poux Martine,
Bertrand Joël
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150111105
Subject(s) - isoelectric focusing , chromatography , chemistry , isoelectric point , flow (mathematics) , range (aeronautics) , analytical chemistry (journal) , materials science , mechanics , biochemistry , physics , composite material , enzyme
Free‐flow isoelectric focusing was adapted to preparative scale separations and chemical engineering methods were used to describe the main mechanisms operating in the apparatus. A mixture of human serum albumin (p I 4.6) and β‐lactoglobulin (p I 5.22) was separated in pH gradients, generated with carrier ampholytes of different origin and covering the pH ranges 4‐6.5, 3.5‐5, 4‐5.5 and 4.5‐5.0. Best results were obtained in the pH 4‐5.5 range. The experimental results have validated the results obtained with a numerical model.