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A general‐purpose pulsed field controller
Author(s) -
Abbal Philippe,
Picou Christian
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150111021
Subject(s) - controller (irrigation) , electrophoresis , voltage , high voltage , transistor , field (mathematics) , materials science , power (physics) , electrical engineering , computer science , analytical chemistry (journal) , optoelectronics , chromatography , physics , chemistry , biology , engineering , mathematics , quantum mechanics , pure mathematics , agronomy
A pulsed field controller compatible with various types of electrophoresis tanks and power supplies is described. The system is based on a high‐precision timing circuit, with an output isolated from the main high voltage supply, and four high voltage transistors. The switching time is programmed manually between 5–160 s. With a transverse alternating field electrophoresis system, intact yeast chromosomes could be completely separated after 24 h.