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Isoelectric focusing in immobilized pH gradients of phosphoglucomutase and esterases from the spiny lobster
Author(s) -
Espinosa Georgina,
Wenisch Elisabeth,
Danner Herbert,
Katinger Hermann,
Righetti Pier Giorgio
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150111006
Subject(s) - phosphoglucomutase , isoelectric focusing , locus (genetics) , biology , chromatography , biochemistry , polyacrylamide gel electrophoresis , chemistry , enzyme , microbiology and biotechnology , gene
A method is described for detecting polymorphisms of cephalothorax and tail homegenates of 25 puerulus staged Panulirus argus in phosphoglucomutase (PGM) a esterases. Isoelectric focusing in immobilized pH gradients was used. In the pH 6.0–8.0 interval for phosphoglucomutase and in the pH 3.5–5.0 and 4.2–4.9 rang for esterases both enzymes appeared as polymorphic band patterns. These could explained by one locus with 2 alleles for phosphoglucomutase and 3 loci with 2, 3 and 4 alleles for esterases. Esterases exhibit a more extensive polymorphism in immobilied pH gradients than in polyacrylamide gel electrophoresis.