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Multiphasic electrophoresis with diversified spacers
Author(s) -
Bringard Aline,
Charlionet Roland
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150111005
Subject(s) - isotachophoresis , electrophoresis , isoelectric focusing , chromatography , chemistry , electrolyte , biochemistry , electrode , enzyme
The principle and some applications of multiphasic electrophoresis with diversified spacers are described. The method combines previously published concepts of introducing highly diversified spacers into the stack of an isotachophoretic system with the theory of snow‐ and telescope‐electrophoresis. The link between this electrophoretic method and isotachophoresis on the one hand and monophasic zone electrophoresis on the other hand is exemplified. The importance of the nature of the spacers as well as of the pH and ions in the leading and terminating zone is illustrated. Details are given concerning the experimental set‐up. This electrophoretic technique could serve as an alternative to isoelectric focusing.