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Capillary zone electrophoresis separation and laser‐based detection of both fluorescein thiohydantoin and dimethylaminoazobenzene thiohydantoin derivatives of amino acids
Author(s) -
Waldron Karen C.,
Wu Shaole,
Earle Colin W.,
Harke Heather R.,
Dovichi Norman J.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110918
Subject(s) - fluorescein , capillary electrophoresis , chromatography , chemistry , electrophoresis , derivative (finance) , detection limit , amino acid , biochemistry , fluorescence , physics , quantum mechanics , financial economics , economics
Capillary zone electrophoresis is employed for the separation and analysis of both fluorescein thiohydantoin and dimethylaminoazobenzene thiohydantoin derivatives of amino acids. Detection of minute amounts of these amino acid derivatives is an important milestone in the development of a high sensitivity protein sequencer. Current detection limits for the fluorescein derivative is on the order of 10 −21 moles whereas detection limits for the dimethylaminoazobenzene derivative is on the order of 10 −16 moles.